2HZ2
The x-ray crystal structure of ferric Synechocystis hemoglobin H117A mutant with a covalent linkage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.874, 47.101, 61.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.000 |
| R-factor | 0.18858 |
| Rwork | 0.185 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.727 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.530 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.143 |
| Number of reflections | 9089 | |
| <I/σ(I)> | 22.4 | 8.1 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 6.15 | 4.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 31% PEG MME 5000, 0.1 M MES, 0.2 M ammonium sulfate, 0.01 M cadmium chloride, 0.01 M sodium hepes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
