2HYU

Human Annexin A2 with heparin tetrasaccharide bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97930
Spacegroup name	P 21 21 2
Unit cell lengths	107.934, 54.301, 68.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.860
Rwork	0.199
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1XJL
RMSD bond length	0.005
RMSD bond angle	0.993
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR (2.5)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.860	4.010	1.860
Rmerge	0.075	0.052	0.349
Number of reflections	32153
<I/σ(I)>	12.6
Completeness [%]	92.6	86.4	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	5	290	1,6 hexanediol, pH 5.0, hanging drop, temperature 290K