2HYG

The Structure of apo-MntR from Bacillus subtilis, Native Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Wavelength(s)	1.1159
Spacegroup name	P 31 2 1
Unit cell lengths	42.000, 42.000, 149.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.28016
Rwork	0.274
R-free	0.34206
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hyf chain D
RMSD bond length	0.009
RMSD bond angle	1.092
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	EPMR
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.500	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.041	0.350
Number of reflections	4162
<I/σ(I)>	8.8	2
Completeness [%]	99.9	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	15% PEG 4000, 0.2 M ammonium sulfate, 5 mM cobalt(II) chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K