2HY6
A seven-helix coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.487, 35.302, 48.985 |
| Unit cell angles | 86.54, 104.28, 99.95 |
Refinement procedure
| Resolution | 47.500 - 1.250 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b1f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.081 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.046 | 0.339 |
| Number of reflections | 51370 | |
| <I/σ(I)> | 12.2 | 2.3 |
| Completeness [%] | 91.8 | 91.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 0.1M potassium dihydrogen phosphate, 1.4M hexanediol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
