2HXW
Crystal Structure of Peb3 from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.472, 101.672, 59.099 |
| Unit cell angles | 90.00, 108.98, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.1883 |
| Rwork | 0.187 |
| R-free | 0.21223 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 69829 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 18% PEG 3350, 0.2M diammonium hydrogen citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
