2HXU

Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-16
Detector	ADSC
Wavelength(s)	0.97911
Spacegroup name	P 6 2 2
Unit cell lengths	159.154, 159.154, 101.961
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.600 - 1.800
R-factor	0.184
Rwork	0.172
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hne
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.600	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	67796
Completeness [%]	96.1	89.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2.0 M ammonium sulfate, 0.1M Hepes , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K