2HXT

Crystal structure of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and D-erythronohydroxamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-15
Detector	ADSC
Wavelength(s)	0.97911
Spacegroup name	P 6 2 2
Unit cell lengths	159.533, 159.533, 102.069
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.800 - 1.700
R-factor	0.18
Rwork	0.179
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hne
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.800	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	82705
Completeness [%]	98.6	93.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	56% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K