2HXR
Structure of the ligand binding domain of E. coli CynR, a transcriptional regulator controlling cyanate metabolism
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97885, 0.97874 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.913, 98.555, 62.236 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.050 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 57793 | |
| <I/σ(I)> | 13.8 | 2.9 |
| Completeness [%] | 89.9 | 65.9 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Sodium formate, 22% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
