2HXB
dCTP deaminase-dUTPase from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0070 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 170.896, 170.896, 170.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.550 |
| Rwork | 0.185 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogh |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.013 | 0.482 |
| Number of reflections | 14069 | |
| <I/σ(I)> | 19.4 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30 % PEG400, 0.2M calcium chloride dihydrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
