2HWY
Structure of PIN domain of human SMG5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.591, 42.534, 65.103 |
| Unit cell angles | 90.00, 95.98, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.750 |
| R-factor | 0.27534 |
| Rwork | 0.271 |
| R-free | 0.32882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2HWW |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.366 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 6552 | |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG 4000, 100 mM citrate pH 5.5 , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
