2HVB
Crystal structure of hypothetical protein PH1083 from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-05-11 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 |
Unit cell lengths | 62.561, 62.561, 115.254 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.470 - 2.500 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dqk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.095 | 0.536 |
Number of reflections | 17504 | |
<I/σ(I)> | 9.9 | 3.3 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 291 | 0.2M Magnesium Chloride, 0.1M Bis-Tris, 25% (w/v) PEG 3350, pH 6.5, MICROBATCH, temperature 291K |