2HUO
Crystal structure of mouse myo-inositol oxygenase in complex with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 210 |
| Detector technology | CCD |
| Collection date | 2005-09-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.7389 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.602, 77.202, 85.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.260 - 2.000 |
| R-factor | 0.20641 |
| Rwork | 0.204 |
| R-free | 0.25627 |
| Structure solution method | SAD from multiple crystals |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.745 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.602 |
| Number of reflections | 20619 | |
| <I/σ(I)> | 18.9 | 4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11.8 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Unbuffered 4.4M sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Unbuffered 4.4M sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Unbuffered 4.4M sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Unbuffered 4.4M sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Unbuffered 4.4M sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
