2HUN
Crystal structure of hypothetical protein PH0414 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-17 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.892, 73.677, 100.222 |
| Unit cell angles | 90.00, 129.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.070 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code: 1R66 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.086 | 0.242 |
| Number of reflections | 49776 | |
| <I/σ(I)> | 10.6 | 3.8 |
| Completeness [%] | 97.5 | |
| Redundancy | 4.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.2 | 291 | 0.2M tri-Sodium Citrate, 20% (w/v) PEG3350, pH 8.2, MICROBATCH, temperature 291K |
