2HU9

X-ray structure of the Archaeoglobus fulgidus CopZ N-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-07
Detector	MARRESEARCH
Wavelength(s)	1.74
Spacegroup name	P 21 21 21
Unit cell lengths	56.253, 64.469, 84.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.160 - 1.780
R-factor	0.206
Rwork	0.205
R-free	0.22100
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.455
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.824
High resolution limit [Å]	1.780	1.740
Rmerge		0.451
Number of reflections	29750
<I/σ(I)>		3.2
Completeness [%]	98.9	93.3
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	100 mM sodium acetate, 200 mM ammonium sulfate, 15-20% PEG 2000 MME, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	100 mM sodium acetate, 200 mM ammonium sulfate, 15-20% PEG 2000 MME, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K