2HU5
Binding of inhibitors by Acylaminoacyl-peptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-18 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.400, 103.334, 168.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.726 - 2.000 |
| R-factor | 0.18363 |
| Rwork | 0.181 |
| R-free | 0.22441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ve6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.726 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.560 |
| Number of reflections | 67288 | |
| <I/σ(I)> | 15.12 | 2.7 |
| Completeness [%] | 89.1 | 53.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 78mM sodium acetate, 0.44mM EDTA, 2.2%PEG 4000, 0.56% beta-octyl-glucoside, 10% DMSO, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
