2HU1
Crystal structure Analysis of Hen Egg White Lyszoyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.827, 78.827, 37.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.730 - 1.630 |
| R-factor | 0.15021 |
| Rwork | 0.147 |
| R-free | 0.22030 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | In-House Strcture |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.275 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2) |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.730 | 1.660 |
| High resolution limit [Å] | 1.629 | 1.629 |
| Rmerge | 0.052 | 0.869 |
| Number of reflections | 15141 | |
| <I/σ(I)> | 10.2 | 2.81 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | 0.1M Sodium Acetate pH 4.8, 7% NaCl, 25% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
