2HTN

E. coli bacterioferritin in its as-isolated form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 3
Unit cell lengths	167.893, 167.893, 167.893
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.340 - 2.500
R-factor	0.17243
Rwork	0.171
R-free	0.18803
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bcf
RMSD bond length	0.009
RMSD bond angle	1.162
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.540	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	69835
Completeness [%]	99.8	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30% (v/v) PEG400, 0.2M MGCl2, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K