2HTB
Crystal Structure of a putative mutarotase (YeaD) from Salmonella typhimurium in monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.553, 47.599, 138.086 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 27.800 - 2.500 |
| R-factor | 0.19135 |
| Rwork | 0.189 |
| R-free | 0.23974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | monomer of orthorhombic form of stYeaD (PDB code 2HTA) |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.314 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.443 |
| Number of reflections | 50200 | |
| <I/σ(I)> | 16.4 | 2.3 |
| Completeness [%] | 1.0 | 0.919 |
| Redundancy | 22.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 300 | 15-25% PEG8K, 0.1M citrate pH 5.5, 0.2-0.5mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
