2HT6
Crystal structure of Human Gem G-domain bound to GDP
Experimental procedure
| Experimental method | SAD - inverse beam |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-12-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.843, 81.433, 124.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.282 |
| Number of reflections | 16469 | |
| <I/σ(I)> | 21.8 | 9.2 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% PEG 8000, 0.1 M HEPES, 0.1 M sodium chloride, 0.5 mM magnesium chloride, 1 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
