2HSZ
Crystal structure of a predicted phosphoglycolate phosphatase (hs_0176) from haemophilus somnus 129pt at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97925,1.28281,0.97914 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.617, 118.436, 66.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.988 - 1.900 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.495 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.988 | 29.990 | 1.950 |
| High resolution limit [Å] | 1.897 | 8.500 | 1.900 |
| Rmerge | 0.074 | 0.050 | 0.653 |
| Total number of observations | 4406 | 13790 | |
| Number of reflections | 51412 | ||
| <I/σ(I)> | 6.9 | 9.7 | 1 |
| Completeness [%] | 100.0 | 97.6 | 100 |
| Redundancy | 4.8 | 6.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 277 | 0.2M NaAcetate, 20.0% PEG-3350, No Buffer pH 7.9, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
