2HSN

Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 43 21 2
Unit cell lengths	94.480, 94.480, 88.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.228
Rwork	0.225
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CHAIN C FROM PDB ENTRY 2HQT
RMSD bond length	0.010
RMSD bond angle	1.267
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.056	0.263
Number of reflections	20772
<I/σ(I)>	21.5	4.96
Completeness [%]	99.3	100
Redundancy	11	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	293	8 - 14 % PEG 20.000, 1 - 3 % dioxane, 100 mM BICINE, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 9.00
1		9	293	8 - 14 % PEG 20.000, 1 - 3 % dioxane, 100 mM BICINE, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 9.00