2HSM
Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-23 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.380, 87.460, 52.800 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 52.700 - 3.000 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2HRA PDB ENTRY 2HQT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 7417 | |
| <I/σ(I)> | 13.5 | 3.67 |
| Completeness [%] | 97.7 | 79.7 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 293 | 30-35 % PEG 3350, 0.3-0.5 M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.20 |
