2HSJ
The structure of a putative platelet activating factor from Streptococcus pneumonia.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926, 0.97943 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.032, 120.063, 84.743 |
| Unit cell angles | 90.00, 99.48, 90.00 |
Refinement procedure
| Resolution | 40.460 - 1.500 |
| R-factor | 0.16161 |
| Rwork | 0.160 |
| R-free | 0.19311 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 128174 | |
| Completeness [%] | 99.4 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M MgCl2, 0.1M Tris, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
