2HRV
2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 72.921, 72.921, 161.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.950 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.053 * | |
| Total number of observations | 109005 * | |
| Number of reflections | 36626 | |
| <I/σ(I)> | 15.6 | 2.4 |
| Completeness [%] | 98.5 | 96.6 |
| Redundancy | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 4 * | pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 7.5 (%) | |
| 4 | 1 | reservoir | 0.3 (M) | ||
| 5 | 1 | reservoir | tertiary amyl alcohol | 5 (%(v/v)) | |
| 6 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 7 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 8 | 1 | reservoir | sodium potassium phosphate | 50 (mM) |
