2HRG
Crystal Structure of Blue Laccase from Trametes trogii complexed with p-methylbenzoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.385, 85.128, 108.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.330 - 1.580 |
| R-factor | 0.17467 |
| Rwork | 0.174 |
| R-free | 0.19215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Blue Laccase from Panus tigrinus (not yet deposited) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.382 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.130 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.059 | 0.320 |
| Number of reflections | 106985 | |
| <I/σ(I)> | 15.1 | 3.2 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | PEG 8000, calcium acetate, PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
