2HR3
Crystal structure of putative transcriptional regulator protein from Pseudomonas aeruginosa PA01 at 2.4 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97954, 0.97970 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.251, 72.281, 70.043 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.21191 |
| Rwork | 0.208 |
| R-free | 0.28021 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.680 | 2.420 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.085 | 0.540 |
| Number of reflections | 22942 | |
| <I/σ(I)> | 31.3 | 2.2 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 8.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296.2 | 0.2M di-Ammonium hydrogen citrate, 20% PEG 3350, 500mM Sodium chloride, 10mM HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.2K |
