2HR2

Crystal structure of a tpr-like protein (ct2138) from chlorobium tepidum tls at 2.54 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97936, 0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	89.090, 91.280, 176.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.696 - 2.540
R-factor	0.205
Rwork	0.204
R-free	0.23200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.171
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.696		2.630
High resolution limit [Å]	2.540	4.330	2.540
Rmerge	0.073	0.044	0.563
Number of reflections	47660
<I/σ(I)>	8.08	15	1.8
Completeness [%]	90.9	96.2	80.6
Redundancy	2.18		2.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	7.5	277	25.0% Glycerol, 0.6M KH2PO4, 0.6M NaH2PO4, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K