2HQS
Crystal structure of TolB/Pal complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-03-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.847, 89.049, 91.051 |
| Unit cell angles | 87.15, 89.62, 68.81 |
Refinement procedure
| Resolution | 38.320 - 1.500 |
| R-factor | 0.18201 |
| Rwork | 0.180 |
| R-free | 0.21267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.680 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.044 | 0.266 |
| Number of reflections | 334414 | |
| <I/σ(I)> | 8.7 | 1.9 |
| Completeness [%] | 95.4 | 94.1 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | MME PEG 2000, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
