2HQM

Crystal Structure of Glutathione Reductase Glr1 from the Yeast Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-11-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 42
Unit cell lengths	116.966, 116.966, 85.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.050 - 2.400
R-factor	0.202
Rwork	0.202
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dnc
RMSD bond length	0.015
RMSD bond angle	1.800
Data reduction software	AUTOMAR
Phasing software	X-PLOR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.550
High resolution limit [Å]	2.400	2.400
Number of reflections	41894
Completeness [%]	92.8	84.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.3	289	10% PEG 6000, 0.1M MES pH 5.3, EVAPORATION, temperature 289K