2HQF
Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-08 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | H 3 2 |
Unit cell lengths | 145.648, 145.648, 519.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.380 |
R-factor | 0.2522 |
Rwork | 0.251 |
R-free | 0.27967 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.078 |
RMSD bond angle | 6.042 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.380 | 3.380 |
Number of reflections | 879227 | |
Completeness [%] | 99.9 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |