2HQE

Crystal structure of human P100 Tudor domain: Large fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	49.931, 93.413, 95.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.240 - 2.000
R-factor	0.23427
Rwork	0.233
R-free	0.24919
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hqx
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	29102
<I/σ(I)>	12.7	12.1
Completeness [%]	89.1	100
Redundancy	8.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	291	2 micor liter drops containing equal volumes of protein solution (10.0 mg/ml) and precipitate solution (20% PEG 8000, 100.0 MM Phosphate, 0.1M Hepes), pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K