2HPZ
Crystal structure of proteinase K complex with a synthetic peptide KLKLLVVIRLK at 1.69 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.312, 68.312, 108.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.690 |
| R-factor | 0.15983 |
| Rwork | 0.159 |
| R-free | 0.17944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p7v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.244 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 29433 | |
| <I/σ(I)> | 9.5 | 2.3 |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 50mM TRIS, 10mM CaCl2, 1M NaNO3, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
