2HPV
Crystal structure of FMN-Dependent azoreductase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2005-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.038, 99.629, 106.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.780 - 2.000 |
| R-factor | 0.20183 |
| Rwork | 0.199 |
| R-free | 0.24952 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.660 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SCA2STRUCTURE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 64925 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 96.3 | 65.6 |
| Redundancy | 8.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | CRYSTALS WERE GROWN BY HANGING DROP VAPOR DIFFUSION USING 1 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN SOLUTION (10 MG/ML) AND A PRECIPITANT SOLUTION CONTAINING 0.09M HEPES/NAOH BUFFER, 1.26M TRI-SODIUM CITRATE DIHYDRATE, 10% V/V GLYCEROL, pH 7.5, temperature 293K |
