2HPL
Crystal structure of the mouse p97/PNGase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.04 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.608, 52.420, 35.028 |
| Unit cell angles | 90.00, 115.33, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.146 |
| R-free | 0.18500 |
| Structure solution method | REFMAC 5.2.0005 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.479 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2.0005) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.314 |
| Number of reflections | 8764 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 87.7 | 46.1 |
| Redundancy | 5.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 281 | 18-22% PEG 5000 MME and 14-18% Tacsimate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
