2HP6
Crystal structure of the OXA-10 W154A mutant at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.600, 96.720, 125.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.400 - 2.200 |
| R-factor | 0.17054 |
| Rwork | 0.168 |
| R-free | 0.21133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1K4F |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.298 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | 0.401 |
| Number of reflections | 30362 | |
| <I/σ(I)> | 15.1 | 5.5 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2M Ammonium sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
