2HOF
Crystal structure of the pre-cleavage synaptic complex in the cre-loxp site-specific recombination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.078 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.625, 122.130, 178.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.960 - 2.400 |
| R-factor | 0.24 |
| Rwork | 0.209 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.452 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.397 |
| Number of reflections | 45638 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.1 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | MPD, SODIUM ACETATE, CALCIUM CHLORIDE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | SODIUM ACETATE | ||
| 3 | 1 | 1 | CALCIUM CHLORIDE | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | SODIUM ACETATE | ||
| 6 | 1 | 2 | CALCIUM CHLORIDE | ||
| 7 | 1 | 2 | MPD |
