2HO2

Structure of human FE65-WW domain in complex with hMena peptide.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	95
Detector technology	CCD
Collection date	2006-06-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.9793, 0.96
Spacegroup name	P 31 2 1
Unit cell lengths	41.661, 41.661, 38.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.030 - 1.330
R-factor	0.174
Rwork	0.172
R-free	0.21400
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.958
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.380
High resolution limit [Å]	1.330	2.870	1.330
Rmerge	0.057	0.051	0.438
Number of reflections	9129
<I/σ(I)>	23.5
Completeness [%]	98.6	99.5	90.3
Redundancy	6.5	6.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	1.85M Ammonium Sulfate, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K