2HNV

Crystal Structure of a Dipeptide Complex of the Q58V Mutant of Bovine Neurophysin-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.541
Spacegroup name	P 62 2 2
Unit cell lengths	110.904, 110.904, 126.682
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.040 - 2.500
Rwork	0.218
R-free	0.28200
Starting model (for MR)	2hnu
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.660	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.074	0.041	0.678
Number of reflections	17407
<I/σ(I)>	15.8
Completeness [%]	99.6	97.4	100
Redundancy	8.2	8.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	0.2 M calcium chloride dihydrate, 0.1 M sodium acetate trihydrate, 22% v/v isopropanol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K