2HNE
Crystal structure of l-fuconate dehydratase from xanthomonas campestris pv. campestris str. ATCC 33913
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 130.633, 130.633, 195.829 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.680 - 2.000 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yey |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.680 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 122876 | |
| Completeness [%] | 94.1 | 91.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 25% PEG 3350, 0.2 M MAGNESIUM FORMATE, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
