2HMY
BINARY COMPLEX OF HHAI METHYLTRANSFERASE WITH ADOMET FORMED IN THE PRESENCE OF A SHORT NONPSECIFIC DNA OLIGONUCLEOTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.300, 74.680, 90.960 |
| Unit cell angles | 90.00, 103.00, 90.00 |
Refinement procedure
| Resolution | 34.500 - 2.610 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1hmy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.880 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.500 |
| High resolution limit [Å] | 2.610 |
| Number of reflections | 20104 |
| Completeness [%] | 91.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 2.2M AMMONIUM SULFATE 50 MMSODIUM CITRATE PH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 2 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 3 | 1 | drop | protein | 0.175 (M) |
