2HMW
Square-shaped octameric ring structure of an RCK domain with ATP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Collection date | 2005-09-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 126.280, 126.280, 98.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.268 |
| Rwork | 0.264 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | single domain from pdb entry 1LSU |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.312 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.731 |
| Number of reflections | 8231 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 9.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 500mM MgCl2, 50mM Tris pH 8.8, 8.5% (w/v) PEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
