2HMB

THREE-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN MUSCLE FATTY ACID-BINDING PROTEIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	35.400, 56.700, 72.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 2.100
R-factor	0.195
RMSD bond length	0.013
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.052 *
Total number of observations	35568 *
Number of reflections	7804 *
Completeness [%]	90.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	PIPES	20 (mM)
3	1	drop	sodium azide	0.05 (%)
4	1	drop	PEG4000	30 (%)
5	1	drop	PIPES	20 (mM)
6	1	drop	sodium azide	0.05 (%)
7	1	reservoir	PEG4000	30 (%)
8	1	reservoir	PIPES	20 (mM)
9	1	reservoir	sodium azide	0.05 (%)