2HLY

The crystal structure of genomics APC5867

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-04-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	2.29
Spacegroup name	P 21 21 21
Unit cell lengths	44.555, 50.391, 81.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.840 - 1.600
R-factor	0.17435
Rwork	0.173
R-free	0.21352
Structure solution method	Sulfur SAD
RMSD bond length	0.010
RMSD bond angle	1.372
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.037	0.100
Number of reflections	23511
<I/σ(I)>	30	9.4
Completeness [%]	94.5	57
Redundancy	6.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	297	0.1M Bis-Tris pH 6.0, 1.5M Ammonium Sulphate, VAPOR DIFFUSION, temperature 297K
1	VAPOR DIFFUSION	6	297	0.1M Bis-Tris pH 6.0, 1.5M Ammonium Sulphate, VAPOR DIFFUSION, temperature 297K