2HLR
Crystal Structure of the Extracellular Domain of the Type II BMP Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 35.063, 35.063, 121.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.290 - 1.200 |
| R-factor | 0.22061 |
| Rwork | 0.220 |
| R-free | 0.22910 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.214 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.290 | 1.232 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.091 | 0.585 |
| Number of reflections | 23947 | |
| <I/σ(I)> | 15.2 | 13.6 |
| Completeness [%] | 98.1 | 88.2 |
| Redundancy | 29.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.1M Na Citrate, 25% PEG2000 MME, 0.8M Sodium Formate, pH 6.0, vapor diffusion, hanging drop, temperature 290K |
