2HLD
Crystal structure of yeast mitochondrial F1-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.524, 294.132, 190.432 |
| Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.20731 |
| Rwork | 0.207 |
| R-free | 0.24412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e1q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.346 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 | 0.406 |
| Number of reflections | 346124 | |
| <I/σ(I)> | 11.7 | 2.6 |
| Completeness [%] | 94.0 | 94 |
| Redundancy | 3.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 5.5% PEG 6000, 10% glycerol, 4% methanol, 0.05M sodium acetate, 0.5mM nickel sulphate, 0.5mM AMP/PNP, 0.025 mM ADP, 2mM magnesium chloride, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
