2HL8
SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfinic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.936, 40.189, 55.371 |
| Unit cell angles | 90.00, 112.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.000 |
| Rwork | 0.192 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euv |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.912 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.630 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.359 |
| Total number of observations | 10146 | |
| Number of reflections | 14290 | |
| <I/σ(I)> | 16.3 | 5.1 |
| Completeness [%] | 97.2 | 95.5 |
| Redundancy | 7.35 | 7.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20%(w/v) PEG 3350, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
