2HL7

Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97920, 0.97940, 0.97560
Spacegroup name	P 21 21 21
Unit cell lengths	40.073, 45.414, 48.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.21065
Rwork	0.209
R-free	0.23493
Structure solution method	MAD, MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.358
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	10212
Completeness [%]	97.7	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	20% PEG 1000, 0.1M Tris-HCl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	20% PEG 1000, 0.1M Tris-HCl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	20% PEG 1000, 0.1M Tris-HCl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K