2HJM
Crystal structure of a singleton protein PF1176 from P. furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.680, 64.240, 111.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27890 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SCA2STRUCTURE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.649 |
| Number of reflections | 12787 | |
| <I/σ(I)> | 12.1 | 3.53 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 14 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.2 | 298 | 100mM Na3 Citrate pH 5.2, 30% PEG400, VAPOR DIFFUSION, temperature 298K |
