2HJD

Crystal structure of a second quorum sensing antiactivator TraM2 from A. tumefaciens strain A6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	298
Detector technology	CCD
Collection date	2004-10-31
Detector	ADSC QUANTUM 4
Spacegroup name	P 43
Unit cell lengths	79.521, 79.521, 90.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.100
Rwork	0.229
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rfy
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	32632
Completeness [%]	99.4	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	50 mM TrisHCl, 200 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 25% ethylene glycol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K