2HJ6
Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylserine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.355, 139.355, 183.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.290 - 3.000 |
| R-factor | 0.17683 |
| Rwork | 0.174 |
| R-free | 0.22719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Unpublished structure of reaction centre at 1.95A resolution |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.290 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.085 | 0.575 |
| Number of reflections | 41533 | |
| <I/σ(I)> | 15.3 | 3.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Potassium phosphate, LDAO, 1,2,3-heptanetriol, 1,2,3-hexanetriol, dioxane, NaCl, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 16.0K |
